固定化药靶蛋白中药活性成分定向筛选研究进展

寸思迪; 张子龙; 王云杉; 龙凯花; 赵雪; 王静; 李倩; 赵新锋

doi:10.16152/j.cnki.xdxbzr.2024-05-008

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固定化药靶蛋白中药活性成分定向筛选研究进展

西北大学生物学科办学100周年专刊 | 更新时间：2024-09-30

- 固定化药靶蛋白中药活性成分定向筛选研究进展
- Research progress on targeted screening of bioactive compounds from traditional Chinese medicine by immobilized target proteins
- 西北大学学报（自然科学版） 2024年54卷第5期页码：858-866
- 作者机构：
  
  西北大学　生命科学学院/西部资源生物与现代生物技术教育部重点实验室，陕西　西安　710069
- 作者简介：
  
  寸思迪，男，博士生，从事药物发现及药物分析方法研究，sidicun0411@163.com。
  赵新锋，男，博士，教授，博士生导师，从事药物分析新方法的创建及其应用研究，zhaoxf@nwu.edu.cn。
- 基金信息：
  
  国家自然科学基金(22374116;22074118)
- DOI：10.16152/j.cnki.xdxbzr.2024-05-008
  中图分类号： R917
- 纸质出版日期：2024-10-25，
  
  收稿日期：2024-08-10，
- 稿件说明：
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寸思迪, 张子龙, 王云杉, 等. 固定化药靶蛋白中药活性成分定向筛选研究进展[J]. 西北大学学报（自然科学版）, 2024,54(5):858-866.

CUN Sidi, ZHANG Zilong, WANG Yunshan, et al. Research progress on targeted screening of bioactive compounds from traditional Chinese medicine by immobilized target proteins[J]. Journal of Northwest University (Natural Science Edition), 2024,54(5):858-866.
寸思迪, 张子龙, 王云杉, 等. 固定化药靶蛋白中药活性成分定向筛选研究进展[J]. 西北大学学报（自然科学版）, 2024,54(5):858-866. DOI： 10.16152/j.cnki.xdxbzr.2024-05-008.

CUN Sidi, ZHANG Zilong, WANG Yunshan, et al. Research progress on targeted screening of bioactive compounds from traditional Chinese medicine by immobilized target proteins[J]. Journal of Northwest University (Natural Science Edition), 2024,54(5):858-866. DOI： 10.16152/j.cnki.xdxbzr.2024-05-008.

摘要

中药活性成分筛选及作用评价一直是破解药物发现周期长和成功率低等瓶颈问题的核心突破口和重要技术支撑。作者团队创新亲和色谱原理，将药靶蛋白定向固定于色谱担体表面，制备了系列高活性功能化色谱固定相，并提出了固定化药靶蛋白定向筛选中药功效成分的新观点。主要综述了作者团队近五年在固定化药靶蛋白色谱固定相制备技术、中药活性成分定向筛选技术以及活性成分早期成药性评价色谱技术等方面的工作，并对固定化药靶蛋白在中药活性成分定向筛选方面的研究进行了展望。

Abstract

Drug discovery has always faced the problems such as long cycles and low success rates. The screening and evaluation of the potential compounds from traditional Chinese medicine have become the core breakthrough point and important technical support to solve the above problems. The authors’ group innovated the principles of affinity chromatography. They immobilized several target proteins onto solid supports

prepared a series of highly active functional chromatographic stationary phases

and proposed the new concept of targeted screening of bioactive compounds from traditional Chinese medicine by immobilized target proteins. The paper reviews the work from the authors’ group during the last five years

which included the methods for the oriented protein immobilization

the targeted screening approaches for bioactive compounds from traditional Chinese medicine

and the chromatographic methods for the early evaluation of potential compounds. Finally

the potential application of the immobilized target proteins in the targeted screening of bioactive compounds from traditional Chinese medicine were also summarized.

关键词

药靶蛋白定向固定中药活性成分定向筛选早期成药性评价

Keywords

immobilized target proteinsbioactive compounds from traditional Chinese medicinetargeted screeningearly evaluation of the potential compounds

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